Activation of stress-responsive mitogen-activated protein kinase pathways in hybrid poplar (Populus trichocarpa x Populus deltoides).
Identifieur interne : 004115 ( Main/Exploration ); précédent : 004114; suivant : 004116Activation of stress-responsive mitogen-activated protein kinase pathways in hybrid poplar (Populus trichocarpa x Populus deltoides).
Auteurs : Louis-Philippe Hamel [Canada] ; Godfrey P. Miles ; Marcus A. Samuel ; Brian E. Ellis ; Armand Séguin ; Nathalie BeaudoinSource :
- Tree physiology [ 0829-318X ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- antagonistes et inhibiteurs : Chitosane.
- enzymologie : Arbres, Populus.
- métabolisme : Mitogen-Activated Protein Kinases.
- pharmacologie : Chitosane, Ozone.
- physiologie : Arbres, Populus.
- Activation enzymatique, Technique de Western.
English descriptors
- KwdEn :
- MESH :
- chemical , antagonists & inhibitors : Chitosan.
- chemical , metabolism : Mitogen-Activated Protein Kinases.
- chemical , pharmacology : Chitosan, Ozone.
- enzymology : Populus, Trees.
- physiology : Populus, Trees.
- Blotting, Western, Enzyme Activation.
Abstract
Plant mitogen-activated protein kinase (MAPK) cascades are important amplifying modules that can rapidly transduce stress signals into various appropriate intracellular responses. Several extracellular regulated kinase (ERK)-type MAPKs involved in plant defense signaling have been identified in herbaceous species, but no MAPK cascade has yet been characterized in a tree species. We examined the signal transduction events that lead to activation of defense mechanisms in poplar, a major forest species of economic and ecological importance which is becoming the model tree system for studying stress and adaptation responses. We show that, in poplar cell suspensions and leaf tissue, chitosan, a non-host-specific elicitor, and ozone, a strong oxidant and atmospheric pollutant, induce rapid and transient activation of at least two myelin basic protein (MBP) kinases with apparent molecular masses of 44 and 47 kD. The chitosan- and ozone-activated kinases have characteristics of MAPKs-they preferentially phosphorylate MBP, require tyrosine and threonine phosphorylation to be activated and are specifically recognized by anti-ERK and anti-pERK antibodies. Moreover, activation of these poplar MAPKs by chitosan or ozone is dependent on the production of reactive oxygen species; the influx of calcium ions via membrane channels; the activation of an upstream, membrane-localized component; and a cognate MAPK kinase (MAPKK). These data suggest that biotic and abiotic challenges activate MAPKs in poplar, as in herbaceous species, which then function as a convergence point for pathogen defense and oxidant stress signaling cascades.
DOI: 10.1093/treephys/25.3.277
PubMed: 15631976
Affiliations:
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Le document en format XML
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Miles</name></author><author><name sortKey="Samuel, Marcus A" sort="Samuel, Marcus A" uniqKey="Samuel M" first="Marcus A" last="Samuel">Marcus A. Samuel</name></author><author><name sortKey="Ellis, Brian E" sort="Ellis, Brian E" uniqKey="Ellis B" first="Brian E" last="Ellis">Brian E. Ellis</name></author><author><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Séguin">Armand Séguin</name></author><author><name sortKey="Beaudoin, Nathalie" sort="Beaudoin, Nathalie" uniqKey="Beaudoin N" first="Nathalie" last="Beaudoin">Nathalie Beaudoin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15631976</idno><idno type="pmid">15631976</idno><idno type="doi">10.1093/treephys/25.3.277</idno><idno type="wicri:Area/Main/Corpus">004141</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004141</idno><idno type="wicri:Area/Main/Curation">004141</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004141</idno><idno type="wicri:Area/Main/Exploration">004141</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Activation of stress-responsive mitogen-activated protein kinase pathways in hybrid poplar (Populus trichocarpa x Populus deltoides).</title><author><name sortKey="Hamel, Louis Philippe" sort="Hamel, Louis Philippe" uniqKey="Hamel L" first="Louis-Philippe" last="Hamel">Louis-Philippe Hamel</name><affiliation wicri:level="1"><nlm:affiliation>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, P.O. Box 3800, QC G1V 4C7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, P.O. Box 3800, QC G1V 4C7</wicri:regionArea><wicri:noRegion>QC G1V 4C7</wicri:noRegion></affiliation></author><author><name sortKey="Miles, Godfrey P" sort="Miles, Godfrey P" uniqKey="Miles G" first="Godfrey P" last="Miles">Godfrey P. Miles</name></author><author><name sortKey="Samuel, Marcus A" sort="Samuel, Marcus A" uniqKey="Samuel M" first="Marcus A" last="Samuel">Marcus A. Samuel</name></author><author><name sortKey="Ellis, Brian E" sort="Ellis, Brian E" uniqKey="Ellis B" first="Brian E" last="Ellis">Brian E. Ellis</name></author><author><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Séguin">Armand Séguin</name></author><author><name sortKey="Beaudoin, Nathalie" sort="Beaudoin, Nathalie" uniqKey="Beaudoin N" first="Nathalie" last="Beaudoin">Nathalie Beaudoin</name></author></analytic><series><title level="j">Tree physiology</title><idno type="ISSN">0829-318X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Western (MeSH)</term><term>Chitosan (antagonists & inhibitors)</term><term>Chitosan (pharmacology)</term><term>Enzyme Activation (MeSH)</term><term>Mitogen-Activated Protein Kinases (metabolism)</term><term>Ozone (pharmacology)</term><term>Populus (enzymology)</term><term>Populus (physiology)</term><term>Trees (enzymology)</term><term>Trees (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation enzymatique (MeSH)</term><term>Arbres (enzymologie)</term><term>Arbres (physiologie)</term><term>Chitosane (antagonistes et inhibiteurs)</term><term>Chitosane (pharmacologie)</term><term>Mitogen-Activated Protein Kinases (métabolisme)</term><term>Ozone (pharmacologie)</term><term>Populus (enzymologie)</term><term>Populus (physiologie)</term><term>Technique de Western (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Chitosan</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mitogen-Activated Protein Kinases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Chitosan</term><term>Ozone</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Chitosane</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arbres</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Mitogen-Activated Protein Kinases</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chitosane</term><term>Ozone</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Arbres</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term><term>Enzyme Activation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation enzymatique</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plant mitogen-activated protein kinase (MAPK) cascades are important amplifying modules that can rapidly transduce stress signals into various appropriate intracellular responses. Several extracellular regulated kinase (ERK)-type MAPKs involved in plant defense signaling have been identified in herbaceous species, but no MAPK cascade has yet been characterized in a tree species. We examined the signal transduction events that lead to activation of defense mechanisms in poplar, a major forest species of economic and ecological importance which is becoming the model tree system for studying stress and adaptation responses. We show that, in poplar cell suspensions and leaf tissue, chitosan, a non-host-specific elicitor, and ozone, a strong oxidant and atmospheric pollutant, induce rapid and transient activation of at least two myelin basic protein (MBP) kinases with apparent molecular masses of 44 and 47 kD. The chitosan- and ozone-activated kinases have characteristics of MAPKs-they preferentially phosphorylate MBP, require tyrosine and threonine phosphorylation to be activated and are specifically recognized by anti-ERK and anti-pERK antibodies. Moreover, activation of these poplar MAPKs by chitosan or ozone is dependent on the production of reactive oxygen species; the influx of calcium ions via membrane channels; the activation of an upstream, membrane-localized component; and a cognate MAPK kinase (MAPKK). These data suggest that biotic and abiotic challenges activate MAPKs in poplar, as in herbaceous species, which then function as a convergence point for pathogen defense and oxidant stress signaling cascades.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15631976</PMID><DateCompleted><Year>2007</Year><Month>01</Month><Day>10</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0829-318X</ISSN><JournalIssue CitedMedium="Print"><Volume>25</Volume><Issue>3</Issue><PubDate><Year>2005</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Activation of stress-responsive mitogen-activated protein kinase pathways in hybrid poplar (Populus trichocarpa x Populus deltoides).</ArticleTitle><Pagination><MedlinePgn>277-88</MedlinePgn></Pagination><Abstract><AbstractText>Plant mitogen-activated protein kinase (MAPK) cascades are important amplifying modules that can rapidly transduce stress signals into various appropriate intracellular responses. Several extracellular regulated kinase (ERK)-type MAPKs involved in plant defense signaling have been identified in herbaceous species, but no MAPK cascade has yet been characterized in a tree species. We examined the signal transduction events that lead to activation of defense mechanisms in poplar, a major forest species of economic and ecological importance which is becoming the model tree system for studying stress and adaptation responses. We show that, in poplar cell suspensions and leaf tissue, chitosan, a non-host-specific elicitor, and ozone, a strong oxidant and atmospheric pollutant, induce rapid and transient activation of at least two myelin basic protein (MBP) kinases with apparent molecular masses of 44 and 47 kD. The chitosan- and ozone-activated kinases have characteristics of MAPKs-they preferentially phosphorylate MBP, require tyrosine and threonine phosphorylation to be activated and are specifically recognized by anti-ERK and anti-pERK antibodies. Moreover, activation of these poplar MAPKs by chitosan or ozone is dependent on the production of reactive oxygen species; the influx of calcium ions via membrane channels; the activation of an upstream, membrane-localized component; and a cognate MAPK kinase (MAPKK). These data suggest that biotic and abiotic challenges activate MAPKs in poplar, as in herbaceous species, which then function as a convergence point for pathogen defense and oxidant stress signaling cascades.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hamel</LastName><ForeName>Louis-Philippe</ForeName><Initials>LP</Initials><AffiliationInfo><Affiliation>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, P.O. Box 3800, QC G1V 4C7, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miles</LastName><ForeName>Godfrey P</ForeName><Initials>GP</Initials></Author><Author ValidYN="Y"><LastName>Samuel</LastName><ForeName>Marcus A</ForeName><Initials>MA</Initials></Author><Author ValidYN="Y"><LastName>Ellis</LastName><ForeName>Brian E</ForeName><Initials>BE</Initials></Author><Author ValidYN="Y"><LastName>Séguin</LastName><ForeName>Armand</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Beaudoin</LastName><ForeName>Nathalie</ForeName><Initials>N</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>66H7ZZK23N</RegistryNumber><NameOfSubstance UI="D010126">Ozone</NameOfSubstance></Chemical><Chemical><RegistryNumber>9012-76-4</RegistryNumber><NameOfSubstance UI="D048271">Chitosan</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.24</RegistryNumber><NameOfSubstance UI="D020928">Mitogen-Activated Protein Kinases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D048271" MajorTopicYN="N">Chitosan</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004789" MajorTopicYN="N">Enzyme Activation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020928" MajorTopicYN="N">Mitogen-Activated Protein Kinases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010126" MajorTopicYN="N">Ozone</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>1</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>1</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>1</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15631976</ArticleId><ArticleId IdType="doi">10.1093/treephys/25.3.277</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Beaudoin, Nathalie" sort="Beaudoin, Nathalie" uniqKey="Beaudoin N" first="Nathalie" last="Beaudoin">Nathalie Beaudoin</name><name sortKey="Ellis, Brian E" sort="Ellis, Brian E" uniqKey="Ellis B" first="Brian E" last="Ellis">Brian E. Ellis</name><name sortKey="Miles, Godfrey P" sort="Miles, Godfrey P" uniqKey="Miles G" first="Godfrey P" last="Miles">Godfrey P. Miles</name><name sortKey="Samuel, Marcus A" sort="Samuel, Marcus A" uniqKey="Samuel M" first="Marcus A" last="Samuel">Marcus A. Samuel</name><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Séguin">Armand Séguin</name></noCountry><country name="Canada"><noRegion><name sortKey="Hamel, Louis Philippe" sort="Hamel, Louis Philippe" uniqKey="Hamel L" first="Louis-Philippe" last="Hamel">Louis-Philippe Hamel</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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